Real-Time PCR System

AriaMx

The AriaMx Real-Time PCR System is a fully integrated quantitative PCR amplification, detection, and data analysis system. We have invented a new, more flexible format for precision qPCR optics.

The latest design combines a state-of-the-art thermal cycler, an advanced optical system with LED excitation source, and data analysis software. Continuing the tradition of intuitive software, the instrument now has a touchscreen so plates and analytics can be started with the touch of a finger.

With enhanced performance, the software provides results capturing RDML information required for publishing under the miQE guidelines. Simultaneously, the software is equipped to export images and raw data in multiple formats, allowing results to be viewed in common programs.

FeatureDescription
Excitation Sourcedye specific LEDs per optical module
Detection Sources8 silicon photo-detectors per optical module
Dyes (at launch)

SYBR/FAM 462.5 – 516.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980)

HEX 535.0 – 555.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980)

ROX 585.0 – 610.0nm Detectable Ct for 0.001ng/DNA per reaction (R2> 0.980)

CY3 532.0 – 568.5nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980)

CY5 635.0- 665.0nm Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980)

6 slots, swappable cartridges Custom cartridges, available upon request

Dye SelectionExcitation and emission
Reaction Volume10μl to 30μl
Chemistries SupportedSYBR, Probe, HRM
Thermal SystemSix peltiers made from two ceramic plates with semi-conductor elements, 96 well
Thermal System Temperature Range25.0 – 99.9°C Heating: 6°C/sec Cooling: 3°C/sec Accuracy: ± 0.2°C or better of target
Dynamic Range10 fold
Comparative Quantitation2x (Range 1.3x to 3x), 10x (Range 6.5x to 15x)
MultiplexDifference in Ct between single-plex and multiplex assay wells is <0.7
Sensitivity (FAM)Discriminates between 2 fold populations ranging from 100k to 12 copies with 95% confidence
UniformityCt St Dev (5s/10s) <0.20, 5 colors, SYBR and TM assays
Electrical Power (input)100-240VAC, 50/60Hz, 1100A
Operating Environment18-35°C (65-95°F), 85% non-condensing humidity 7500 feet, max altitude
Weight50 lbs. (23 kg)
Dimensions19.7” W x 18.1“ D x 16.5” H (50cm x 46cm x 42cm)
Sample Containers0.2ml tubes, 96 well plates, strip tubes
Warranty
  • 2 year warranty is standard with the instrument
  • 5 year warranty and service packages available
Onboard Analytics
  • Thermal, physical, interactive (sensors) tests
  • Extended: 125 performance points tested in 30 minutes
  • Start-up: 59 performance points tested in ~1 minute
  • Optional bypass of both features